The Bio-Reader300 multi-purpose chemiluminescence immunoassay fluorescent microplate reader is a high performance multi-mode detection instrument to provide, fluorescence-based DNA/RNA quantification assays.
The Bio-Reader300 multi-purpose chemiluminescence immunoassay fluorescent microplate reader offers affordable high performance detection solutions, powered by monochromator and filter based technologies.
The Bio-Reader300 multi-purpose microplate reader is a high performance multi-mode detection instrument to provide a simple means of detecting advanced chemistries. This instrument provides researchers UV-visible absorbance measurements, flexible use of filters for fluorescence intensity and filtered luminescence measurements.
· UV / Vis Absorbance
Wavelength selection in Bio-Reader300 is done by using an advanced monochromator system. Any wavelength between 200 to 1000nm can be selected. Using the spectral scanning feature, the whole spectrum of a sample be scanned in 1nm increments to allow identification of the optimal measurement wavelength for a new assay.
Bio-Reader300 is equipped with filter-based fluorescence optics and dichroic mirrors for screening applications such as fluorescence polarization, and TR-FRET. Standard applications such as fluorescence-based DNA/RNA quantification assays are not only supported in microplates, but also in low volume u-Nano Ultra-Micro plate.
The independent removable filter modules make it more convenient for users to replace the filter. The filter-based fluorescence optics detection ensures high sensitivity, greater light transmission, precise control over transmitted peak shape, excellent blockage of undesired wavelengths. This is ideal for excitation and emission applications. The filters are also the technically preferred and most cost efficient technology for non-absorbance based assays.
Bio-Reader300 has been designed with a dedicated luminescence detection system for both flash and glow based assays. It can offer excellent sensitivity and wide dynamic range in luminescence measurement. The optimized precision dual-channel injectors ensure the detection performance of the 96-well and 384-well plate. The PMT enhances the maximum sensitivity of weak luminescence signals, prevents oversaturation of high signals, effectively improves the detection range of luminescence and minimizes signal crosstalk between wells.
· User Authority Classification
User permissions are divided into four levels, with clear permissions to avoid the occurrence of misuse by researchers;
Administrator can manage the accounts of different sub-users;
The users have independent accounts and passwords to ensure the safety and confidentiality of experimental results.
· Intuitive Interface Display
Intuitive selection of function modes, easy parameter setting;
The layout colors are distinguished clearly, convenient to be checked for the position of the sample;
Programs and results are stored independently, making it easy to be found the required applications.
· Powerful Data Analysis and Process
Provide multiple data processing methods including blank subtraction, standard curve creation, qualitative analysis, quality control, kinetics and spectral analysis to help you obtain the analysis results you want;
Algorithm customization: according to your assays needs, you can customize the required algorithm, tailor-made for convenient assays.
· Liberalized User Communication
The software has with a shared library, which can store the program, results and standard curves for sharing them with others;
The program and standard curve can be created in real time to a QR code, and the required content can be imported only by scanning the code with the instrument.
· FTP (File Transfer Protocol)
Upload the data directly to a computer with a FTP server, and users can view the data results at any time in the authorized folder.
|Basic Information||Linear dynamic range||6 logs|
|Function||Absorbance, fluorescence, luminescence||Shaking & Incubation|
|Plate Formats||96 & 384 wells (6/12/24/48 optional)||Shaking mode||Linear, circular, double circular|
|Absorbance||Incubation temperature||RT +4°C to 45°C|
|Light source||High energy xenon flash lamp||Temperature|
|Wavelength accuracy||2nm||Software interface||Chinese/English|
|0.2nm||Screen size||10-inch LCD display|
|Half width (FWHM)||≤2.5nm||Operation method||Touch capacitive screen; use mouse|
|Wavelength range||200-1000nm, 1nm step||Data capacity||10 GB|
|Measuring range||0-4 OD||Compatibility||Support PC software, win7 to win10 64 bit|
|Resolution||0.0001 OD||Data transmission||Test data report can be uploaded to PC|
server via FTP
±(1.0%+0.003Abs) @ (0.0-2.0Abs]
±2.0% @ (2.0-3.0Abs]
|Instrument port||2 USB A type ports / 1 USB B type port|
1 Ethernet port
Rs232 bus interface (sampling device connection)
|Repeatability||CV < 1.0% or SD< 0.003 Fast (0.0-3.0Abs]|
CV < 0.5% or SD< 0.003 Accurate (0.0-3.0Abs]
|Stray light||0.1%@220 nm||Size (WxDxH)||420x550x386mm|
|Linear||R2≥0.999@[0.0-3.0Abs]||Power supply||AC 100 to 240V, 50 to 60Hz|
|Reading time||96-well plate: fast<15s||Weight||33kg|
|Fluorescence||u-Nano Ultra-Micro Plate (Optional)|
|Reading mode||Top reading||Number of samples||1-16|
|Excitation light source||High energy xenon lamp||Sample detection|
|Detector||PMT||Automatic Injector Module (Optional)|
|Wavelength range||EX: 200-1000nm; EM: 270-850nm||Quantity||1, 2|
|Filter EX / EM||3 groups(470nm/525nm,523nm/564nm,|
|5-1000μL, 1μL increment|
|Detection limit||≤1pM||Liquid injection|
|6logs||Accuracy||±1μL@5-50μL ±2% @ 51-1000μL|
|Detector||PMT||PC Software (Optional)|
|Detection limit||15 amol/hole||Analysis software||ReaderIt-II|