Optical path: 1/0.5/0.05mm automatic conversion
Protein range: 0.01-400mg/ml (BSA)
Nucleic acid range: 0.4-15000 ng/μl (DS-DNA)
The K5600 K5600C micro-spectrophotometer, a new type full-spectrum(200~850nm) spectrophotometer, can measure nucleic acids, proteins, cell cultures, micro array samples, and UV-VIS full spectrum absorbance scan with high accuracy and reproducibility. Adding dark room for cuvette, you can choose cuvette or test platform.
Features
0.3~2µL sample size!
Cuvette or test platform!
No warm-up!
Rapid measure speed!
Display the concentration directly!
UV-VIS full spectrum absorbance analysis (200-850nm)!
No dilutions for samples, can measure samples with high concentration!
Easy to use!
Technical data
Path length: 1mm, 0.2mm, (0.1mm, 0.05mm optional)
Sample size: 0.3~2µL
Light source: Xenon flash lamp
Detector: 2048-element linear silicon CCD
Wavelength range: 200~850nm
Cuvette size(path length): 1mm, 2mm, 5mm, 10mm
Wavelength accuracy: ±1nm
Wavelength resolution: 2nm (FWHM at Hg 546nm)
Absorbance precision: 0.002 Abs
Absorbance accuracy: 1% (0.76 absorbance at 350nm)
Absorbance range: 0.002~75(150, 300 optional, 10mm equivalent absorbance)
Detects Nucleic acid up to: 0.4~3750ng/μL(7500,15000 optional, dsDNA)
Detects Protein up to: 0.01mg/ml~100mg/ml(200,400 optional, BSA)
Measurement time: <5s
Outline size: 24cm×21cm×11cm
Net weight: 1.92kg
Application
There are 5 measurement modules provided by K5600 software: Nucleic acid, Protein, Cell culture, Microarray and UV-VIS.
Nucleic Acid: Concentration and purity of DS-DNA, SS-DNA and RNA
Protein: Concentration of the protein A280(1Abs = 1mg/mL), BSA, IgG and lysozyme
Concentration of protein with protein assay kit (Lowry, BCA and Bradford)
Cell Culture:Concentration of suspended cell cultures
UV-VIS: UV/VIS full-spectrum (200~850nm) absorbance scan
Microarray:Concentration of fluorescent dye labeled nucleic acid (microarray), for it can measure the concentration of the nucleic acid and the dye at the same time
General operation
1 Drop directly 0.3~2µL sample onto the test platform with a pipette.
2 Close the sampling arm.
3 Or open the dark room and put the cuvette into it.
4 Click the “Measure” button, and the result(values and graph) of the sample will appear in seconds.
5 Wipe the sample from the test platform with clear absorbent paper.
6 Click the “Save Graphic” button after measured a sample if you want to save the graph.
7 Click the “Show Report” button after the samples measured to output the result data of the samples by excel.
K5600C cuvette parameters
Trial cuvette specifications (light path): 1/2/5/10mm
Cuvette minimum volume: 50μl
Sample level height: 5mm
Type: Recyclable micro cuvette
Cuvette darkroom: standard cuvette darkroom, suitable for multi-size cuvettes